In vitro genebank is an ex-situ conservation method that basically allows safeguarding of valuable genetic diversity of particular crops, outside their original or natural habitat, under artificial growing (in vitro). Achieved with slow growth conditions, in vitro conservation involves maintenance of plant explants in a sterile, pathogen-free environment and mostly applied to vegetative propagated crops that produce non-orthodox, heterogeneous and/or recalcitrant seeds (sometimes do not produce seeds). This modern plant tissue culture technique has already been applied on more than 1000 species for mass propagation (multiplication) or planting material production (micro tubers, nursery), haploidization, micro-grafting (in breeding), bioengineering (genetic transformation) and for rescue of endangered material/species. In general, more economical and less risky in a long-term perspective; as compared to field collections, in vitro genebanking is mainly seen as medium term storage (MTS), with particular culture conditions to slow down the plant growth (slow growth conditions): growth retardation, minimal containment, relatively cold temperature and short photoperiod. In vitro conservation can be associated with long-term storage (LTS), or cryopreservation, at ultra-low temperature using cryogenic techniques (in liquid Nitrogen at -196 o C).
In the in vitro genebank of IITA GRC, both the improved lines and landraces of the above-mentioned crops are conserved for breeding, training, research use and production. The facilities available at the GRC in-vitro genebank includes plant growth and conservation rooms, culture media preparation room, transfer (aseptic handling) room and washing/autoclave room; with all relevant in vitro laboratory relevant equipment (autoclave, laminar flow hoods culture shelves, pH meter, weighing balances, glassware’s, fridges, growth cabinets, water purification system, glass washing machine and dryer). The human resources is composed with well-trained and skillful personnel, competent to carry out in vitro plant tissue culture work in compliances with minimum quality and risk management system(laboratory best practices, safety and security). Order your seeds and plantlet by using this link